KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
조성 | 용량 | 단위 | ||||||||||||||||||||||||
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Trypticase peptone (BD BBL) | 5.0 | g | ||||||||||||||||||||||||
Peptone from meat (pepsin·digested) | 5.0 | g | ||||||||||||||||||||||||
Yeast extract | 10.0 | g | ||||||||||||||||||||||||
Na-Resazurin solution(0.1% w/v) | 0.5 | ml | ||||||||||||||||||||||||
Salt solution (see medium 1477) | 40 | ml | ||||||||||||||||||||||||
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L-Cysteine·HCl ·H2O | 0.5 | g | ||||||||||||||||||||||||
Na2CO3 | 1.0 | g | ||||||||||||||||||||||||
D-Glucose | 5.0 | g | ||||||||||||||||||||||||
Distilled water | 1000 | ml | ||||||||||||||||||||||||
Dissolve ingredients (except cysteine, carbonate and glucose) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add cysteine, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 7.0, if necessary. |
** ph : -
** memo : -
** 추가조치 사항 : For KCTC5071 adjust pH to 6.3.
For KCTC25034 replace glucose with 5g/l gamma-aminobutyrate.
For KCTC25281 replace glucose with 10g/l maltose.
For KCTC25820 Replace glucose with 3.0 g/l Casamino acids.