| Peptone |
0.5 |
g |
| Yeast extract |
0.25 |
g |
| Glucose |
0.1 |
g |
| MES(10mM final concentration) |
1.95 |
g |
| SSE(double concentrated)(see below) |
500 |
ml |
| 조성 |
용량 |
단위 |
| CaCl_2·2H2O |
0.2938 |
g |
| NH4Cl |
0.1069 |
g |
| MgCl_2·6H2O |
0.2036 |
g |
| (NH_4)2SO_4 |
0.1983 |
g |
| MgSO_4·7H2O |
0.7390 |
g |
| CaSO_4·2H2O |
0.8606 |
g |
| Ca(NO_3)_2·4H2O |
0.2360 |
ml |
| NaNO_3 |
0.4240 |
g |
| KH2PO_4 (100mM solution) |
0.5000 |
ml |
| FeSO_4·7H2O |
0.0111 |
g |
| K2SO_4 |
0.0870 |
g |
| add distilled water |
1000 |
ml |
| Dissolve by shaking overnight. Sterilize by autoclaving. |
|
|
|
| Distilled water |
500 |
ml |
| adjust pH to 5.5 |
|
|
| Add to 1000ml of medium after autoclaving: |
|
|
| Trace Element Solution SL-10 |
1.0 |
ml |
| 조성 |
용량 |
단위 |
| HCl (25%; 7.7M) |
10.0 |
ml |
| FeCl_2·4H2O |
1.5 |
g |
| ZnCl_2 |
70 |
mg |
| MnCl_2·4H2O |
100 |
mg |
| H3BO_3 |
6 |
mg |
| CoCl_2·6H2O |
190 |
mg |
| CuCl_2·2H2O |
2.0 |
mg |
| NiCl_2·6H2O |
24 |
mg |
| Na2MoO_4·2H2O |
36 |
mg |
| Distilled water |
990 |
ml |
| First dissolve FeCl_2 in HCl, then dilute with water, add and dissolve the other salts. Finally make up to 1000.0ml. |
|
|
|
| Vitamin solution |
1.0 |
ml |
| 조성 |
용량 |
단위 |
| Biotin |
2.0 |
mg |
| Folic acid |
2.0 |
mg |
| Pyridoxine-HCl |
10.0 |
mg |
| Thiamin-HCl·2H2O |
5.0 |
mg |
| Riboflavin |
5.0 |
mg |
| Nicotinic acid |
5.0 |
mg |
| D-Ca-pantothenate |
5.0 |
mg |
| Vitamin B_12 |
0.1 |
mg |
| p-Aminobenzoic acid |
5.0 |
mg |
| Lipoic acid |
5.0 |
mg |
| Distilled water |
1000 |
ml |
|