Basal medium |
|
|
조성 |
용량 |
단위 |
NaCl |
25 |
g |
NH4Cl |
0.5 |
g |
KH2PO4 |
0.1 |
g |
MgCl2·6H2O |
4 |
g |
CaCl2·2H2O |
1.0 |
g |
NaHCO3 |
2.0 |
g |
Yeast extract |
1.0 |
g |
Sugar |
1.7 |
g |
Trace mineral solution |
1.0 |
ml |
Se/W solution |
1.0 |
ml |
Vitamin solution |
2.0 |
ml |
Resazurin solution (1 mg/ml) |
1.0 |
ml |
Na2S·9H2O |
0.3 |
g |
Cysteine HCl |
0.3 |
g |
distilled water |
1.0 |
L |
|
Trace mineral solution |
|
ml |
조성 |
용량 |
단위 |
FeCl2·4H2O |
1.99 |
g |
CoCl2 |
0.13 |
g |
MnCl2·4H2O |
0.198 |
g |
ZnCl2 |
0.136 |
g |
H3BO3 |
0.0062 |
g |
NiCl2 |
0.013 |
g |
AlCl3 |
0.0133 |
g |
Na2MoO4·2H2O |
0.0242 |
g |
CuCl2·2H2O |
0.0017 |
g |
destilled water |
1.0 |
L |
|
Se/W solution |
|
|
조성 |
용량 |
단위 |
Na2SeO3 |
0.0017 |
g |
Na2WO4·4H2O |
0.0033 |
g |
destilled water |
1.0 |
L |
|
Vitamin solution |
|
|
조성 |
용량 |
단위 |
biotin |
4.88 |
mg |
4-aminobenzoic acid |
2.74 |
mg |
pantothenate (Ca salt) |
9.54 |
mg |
pyridoxine (HCl salt) |
4.12 |
mg |
nicotinamide |
2.44 |
mg |
thiamine (=thioctic acid) |
6.74 |
mg |
lipoic acid |
4.12 |
mg |
folic acid |
8.82 |
mg |
vitamin B12 |
27.1 |
mg |
riboflavin |
7.52 |
mg |
destilled water |
1.0 |
L |
|