KCTC Media No. 1464 : Medium for KCTC 15770
            
                
                | 조성 | 
                용량 | 
                단위 | 
                
                
                
                
                    | milli Q water | 
                    300 | 
                    ml | 
                    
                
                    
                        
                            
                                
                                
                                
                            
                            
                            
                                | 조성 | 
                                용량 | 
                                단위 | 
                             
                            
                            
                                | Glucose | 
                                0.5 | 
                                g | 
                             
                            
                            
                                | Cellobiose | 
                                0.5 | 
                                g | 
                             
                            
                            
                                | K2HPO4 | 
                                37.5 | 
                                ml | 
                             
                            
                            
                                | Na2S·9H2O | 
                                0.25 | 
                                g | 
                             
                            
                            
                                | NaHCO3 | 
                                4 | 
                                g | 
                             
                            
                            
                                | Trypticase | 
                                2.0 | 
                                g | 
                             
                            
                            
                                | Yeast extract | 
                                0.5 | 
                                g | 
                             
                            
                            
                                | Ascorbic acid | 
                                0.5 | 
                                g | 
                             
                            
                            
                                | menadione | 
                                0.002 | 
                                g | 
                             
                            
                            
                                | Resazurin
(25mg resazurin/100ml of dH2O stock solution) | 
                                4 | 
                                ml | 
                             
                            
                            
                                | Histidine Hematin
(1.2 mg hematin/ml in 0.2M histidine (pH 8.0) stock solution) | 
                                1.0 | 
                                ml | 
                             
                            
                            
                                | ATCC Trace Mineral Mix | 
                                37.5 | 
                                ml | 
                             
                            
                            
                                | Cystein·HCl·H2O | 
                                0.25 | 
                                g | 
                             
                            
                            
                                | Filter sterilise | 
                                 | 
                                 | 
                             
                            
                            
                         
                     | 
                
                
                
                
                
                    | milli Q water | 
                    500 | 
                    ml | 
                    
                
                    
                        
                            
                                
                                
                                
                            
                            
                            
                                | 조성 | 
                                용량 | 
                                단위 | 
                             
                            
                            
                                | Agar | 
                                15.0 | 
                                g | 
                             
                            
                            
                                | autoclave, do not let it cool and soiidify | 
                                 | 
                                 | 
                             
                            
                            
                         
                     | 
                
                
                
                
                
                    | milli Q water | 
                    200 | 
                    ml | 
                    
                
                    
                        
                            
                                
                                
                                
                            
                            
                            
                                | 조성 | 
                                용량 | 
                                단위 | 
                             
                            
                            
                                | Soluble starch | 
                                0.5 | 
                                g | 
                             
                            
                            
                                | autoclave, do not let it cool and soiidify | 
                                 | 
                                 | 
                             
                            
                            
                         
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            ** ph : 7
            ** memo : -
            ** 추가조치 사항 : Mix all three components together in anaerobic conditions