KCTC Media No. 1474 : TEPIDANAEROBACTER MEDIUM

조성	용량	단위
KH2PO4	0.14	g
MgCl2·6H2O	0.2	g
CaCl2·2H2O	0.15	g
NH4Cl	0.54	g
Trace element solution(see the medium No.1475)	1.0	ml
Na-resazurin solution (0.1% w/v)	0.5	ml
NaHCO3	2.5	g
Yeast extract	2.3	g
Vitamin solution (see the medium No.1335)	2.0	ml
D-Glucose	2.2	g
Na2S·9H2O	0.3	g
L-Cysteine-HCl·H2O	0.3	g
Distilled water	1000	ml

** ph : 7

** memo : Dissolve ingredients (except bicarbonate, yeast extract, vitamins, glucose and reducing
agents), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 ? 45 min to
make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type
tubes or serum vials and autoclave. Add yeast extract, vitamins (sterilized by filtration),
glucose, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2
gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and
20% CO2 gas mixture. Adjust pH of the complete medium to 7.0-7.2, if necessary.
For DSM 21860 omit glucose, increase amount of ammonium chloride to 2.50 g/l,
decrease amount of yeast extract to 0.20 g/l and supplement medium with 1.00 ml/l
selenite-tungstate solution (see medium 385). As substrate add 1.10 g/l betaine to the
autoclaved medium from an anoxic stock solution sterilized by filtration.

** 추가조치 사항 : -