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미생물클러스터
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MICROBIOTA
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회원가입
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ENG
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Korean Collection for Type Cultures
KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
분양
일반자원검색
KCTC Media No. 1485 : PONTIELLA MEDIUM
| 조성 | 용량 | 단위 |
|---|---|---|
| NaCl | 18 | g/L |
| KCl | 1.5 | g/L |
| Na2CO3 | 1.5 | g/L |
| NH4Cl | 1.0 | g/L |
| K2HPO4 | 0.4 | g/L |
| Na-resazurin solution (0.1% w/v) | 0.5 | ml/L |
| MgCl2·6H2O | 6 | g/L |
| CaCl2·2H2O | 0.4 | g/L |
| Na2S·9H2O | 0.5 | g/L |
| L-Fucose | 0.5 | g/L |
| L-Cysteine-HCl · H2O | 0.5 | g/L |
| Na2SO4 | 1.0 | g/L |
| Modified Wolin’s mineral solution (see medium 918) | 10.0 | ml/L |
| Wolin's vitamin solution (see medium 1335) | 10.0 | ml/L |
| Distilled water | 1.0 | L |
** ph : -
** memo : -
** 추가조치 사항 : Dissolve ingredients except carbonate, fucose, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 ? 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.3 - 7.5, if necessary.
담당자
기탁/분양서류
DNA분양서비스
동결건조서비스
STR분석서비스