KCTC Media No. 1519 : THERMOSULFURIMONAS MEDIUM

조성	용량	단위
NaCl	18	g
MgCl2·6H2O	4	g
KCl	0.33	g
CaCl2·2H2O	0.33	g
(NH4)2SO4	0.5	g
Trace element solution SL-10 (see medium 1417)	1.0	ml
Selenite-tungstate solution (see medium 1418)	1.0	ml
Na2CO3	1.0	g
KH2PO4	0.33	g
Vitamin solution (see medium 1335)	1.0	ml
Na-acetate	1.5	g
Na2S2O3·5H2O	3.5	g
Yeast extract	0.2	g
Distilled water	1.0	L

** ph : 6.5-6.8

** memo : Dissolve ingredients except carbonate, hydrogenphosphate, vitamins, acetate,
thiosulfate and yeast extract, then sparge medium with 80% H2 and 20% CO2 gas
mixture for 30 ? 45 min to make it anoxic. Dispense medium under same gas
atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add
hydrogenphosphate, vitamins, acetate, thiosulfate and yeast extract from sterile anoxic
stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock
solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of thiosulfate
and vitamins should be sterilized by filtration. The pH of the complete medium should be
at 6.5 ? 6.8.
For DSM 29363 prepare medium without acetate, thiosulfate and yeast extract.
Supplement medium after autoclaving with 2.50 g/l Fe(III)-citrate added from a sterile
anoxic stock solution (10% w/v) prepared under 100% N2 gas atmosphere. Adjust pH of
complete medium to 6.0.

** 추가조치 사항 : -