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Distribution
조성 | 용량 | 단위 |
---|---|---|
KH2PO4 | 0.33 | g/L |
NH4Cl | 0.33 | g/L |
KCl | 0.33 | g/L |
MgCl2·6H2O | 0.33 | g/L |
CaCl2·6H2O | 0.33 | g/L |
NaHCO3 | 2.0 | g/L |
NaOH | 0.6 | g/L |
resasurin solution | 0.0002 | % |
The vitamins (Wolin EA, Wolin MJ, Wolfe RS (1963) (See medium 1532) | 1.0 | ml/L |
trace-elements (See medium 1532) | 1.0 | ml/L |
** ph : 6-6.5
** memo : -
** 추가조치 사항 : NaHCO_3_ ,NaOH and vitamins, Na_2_S*9H_2_0 (0.5 g/l) are added after boiling and cooling of the medium under 100% CO_2_.
Adjust the PH between 6.0 and 6.5 with sterile HCl or NaOH after autoclaving.
2g/l glucose is used as a growth substrate and added separately.
Yeast extract is obligatory for growth and at least 50mg/l YE must be added to the final solution befor inoculation.