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Distribution
조성 | 용량 | 단위 |
---|---|---|
Yeast extract | 0.05 | g/L |
Fish Peptone | 0.25 | g/L |
Sodium formate | 0.2 | g/L |
Sodium acetate | 0.2 | g/L |
Sodium lactate | 0.2 | g/L |
Sodium pyruvate | 0.2 | g/L |
Sodium succinate | 0.2 | g/L |
Malic acid | 0.2 | g/L |
Fumaric acid | 0.2 | g/L |
Sodium citrate | 0.2 | g/L |
KCl | 5.4 | g/L |
K2HPO4 | 0.2 | g/L |
CaCl_2 | 0.1 | g/L |
NaNO_3 | 0.5 | g/L |
MgCl_2·6H_2O | 0.1 | g/L |
NaCl | 184 | g/L |
Washed agar | 15-18 | g/L |
Add components to distilled water and bring volume to 1.0 L. Adjust pH to 9.0 with 10% (w/v) Na2CO3. |
** ph : 9.0
** memo : Uploading the HCM 9.0 medium information received from the depositor to the document for KCTC 4304.
** 추가조치 사항 : Washed agar
Add 20.0 g pure agar power to 400 ml distilled water. Mix well for 5 min then allow the agar to settle for 30 min and decant the supernatant. Repeat washing as in the previous step until the supernatant is clear.