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미생물클러스터
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MICROBIOTA
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Korean Collection for Type Cultures
KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
분양
일반자원검색
KCTC Media No. 571 : CHOPPED MEAT MEDIUM WITH CARBOHYDRATES
| 조성 | 용량 | 단위 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Ground beef (fat free) | 500.0 | g | |||||||||
| Distilled water | 1000 | ml | |||||||||
| NaOH 1 N | 25.0 | g | |||||||||
| Casitone | 30.0 | g | |||||||||
| Yeast extract | 5.00 | g | |||||||||
| K2HPO4 | 5.00 | g | |||||||||
| Resazurin | 1.0 | mg | |||||||||
| Glucose | 4.00 | g | |||||||||
| Cellobiose | 1.00 | g | |||||||||
| Maltose | 1.00 | g | |||||||||
| Starch (soluble) | 1.00 | g | |||||||||
| To make medium anoxic boil it, cool under nitrogen atmosphere, add 0.5 g/l cysteine hydrochloride and adjust pH to 7.0. Dispense anoxically 7 ml medium into Hungate tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121?C for 30 min. For agar slants use 15 g agar per 1000.0 ml medium. In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vit | |||||||||||
| Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: | |||||||||||
| In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving | |||||||||||
| Haemin solution (see below) | 10.0 | ml | |||||||||
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| Vitamin K1 or Vitamin K3 solution (see below) | 10.0 | ml | |||||||||
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** ph : -
** memo : To medium 18 add 0.4% glucose, 0.1% cellobiose, 0.1% maltose, and 0.1% soluble starch before adding cysteine.
** 추가조치 사항 : -
담당자
기탁/분양서류
DNA분양서비스
동결건조서비스
STR분석서비스