| Solution A: |
|
|
| 조성 |
용량 |
단위 |
| KCl |
1.70 |
g |
| CaCl2 . 2H2O |
1.25 |
g |
| KH2PO4 |
1.70 |
g |
| NH4Cl |
1.70 |
g |
| MgSO4 |
2.50 |
g |
| Distilled water |
4000.00 |
ml |
| (For marine or estuarine isolates add 100.0 g NaCl to this solution and increase the MgSO4 . 7H2O
to 15.0 g). |
|
|
|
| Solution B: |
|
|
| 조성 |
용량 |
단위 |
| Distilled water |
860.00 |
ml |
| Autoclave in a cotton-stoppered Erlenmeyer flask and cool to room temperature under an atmosphere of
N2 in an anaerobic jar. |
|
|
|
| Solution C: |
|
|
| 조성 |
용량 |
단위 |
| Vitamin B12 solution (0.002% in H2O) |
5.00 |
ml |
| Filter sterilize. |
|
|
|
| Solution D: |
|
|
| 조성 |
용량 |
단위 |
| Trace element solution (SL-12 B) |
5.00 |
ml |
| Autoclave at 121°C for 15 min. |
|
|
|
| Solution E: |
|
|
| 조성 |
용량 |
단위 |
| NaHCO3 |
7.50 |
g |
| H2O |
100.00 |
ml |
| Bubble with CO2 and, after saturation, filter sterilize under CO2 pressure into sterile, gas-tight,
100 ml screw-cap bottle. |
|
|
|
| Solution F: |
|
|
| 조성 |
용량 |
단위 |
| Na2S . 9H2O (10 g in 100 ml) |
20.00 |
ml |
| Prepare in a screw-cap bottle, bubble with N2 to replace air, close tightly and autoclave. |
|
|
|
| Trace element solution SL-12 B: |
|
|
| 조성 |
용량 |
단위 |
| Distilled water |
1000.00 |
ml |
| Adjust pH to 6.0.
Autoclave solution A for 45 min. in 5-litre special bottle or flask (with four openings at the top)
at 121°C, together with a teflon-coated magnetic bar. In this 5-litre bottle, two openings for tubes
are in the central, silicon rubber stopper; a short, gas-inlet tube with a sterile cotton filter;
and an outlet tube for medium, which reaches the bottom of the vessel at one end and has, at the
other end, a silicon rubber tube with a pinch cock and a bell for aseptic dispensing of the medium
into bottles. The other two openings have gas-tight screw caps; one of these openings is for the
addition of sterile solutions and the other serves as a gas outlet.
After autoclaving cool solution A to room temperature under a N2 atmosphere with a positive pressure
of 0.05 - 0.1 atm (a manometer for low pressure will be required). Saturate the cold medium with CO2
by magnetic stirring for 30 min. under a CO2 atmosphere of 0.05 - 0.1 atm. Add solution B, C, D, E
and F th |
|
|
| Na2-EDTA |
3.00 |
g |
| FeSO4 . 7H2O |
1.10 |
g |
| CoCl2 . 6H2O |
190.00 |
mg |
| MnCl2 . 2H2O |
50.00 |
mg |
| ZnCl2 |
42.00 |
mg |
| NiCl2 . 6H2O |
24.00 |
mg |
| CuCl2 . 2H2O |
2.00 |
mg |
| H3BO3 |
300.00 |
mg |
| Na2MoO4 . 2H2O |
18.00 |
mg |
|
미생물클러스터
MICROBIOTA
회원가입
ENG
담당자
기탁/분양서류
DNA분양서비스
동결건조서비스
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