KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
조성 | 용량 | 단위 |
---|---|---|
Trypticase Soy Broth (BBL no. 11774) | 5.5 | g |
Resazurin (0.1% w/v) | 0.5 | ml |
D-Glucose | 2.0 | g |
Distilled water | 1.0 | L |
Dissolve dehydrated TSB medium and resazurin, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Adjust pH to 5.5, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a separately sterilized anoxic stock solution. |
** ph : -
** memo : Prepare TSB anaerobically under 100% N_2_. Adjust pH to 5.5 prior to dispensing and autoclaving. Add glucose from separately sterilized, anaerobic 20% (w/v) solution.
** 추가조치 사항 : For KCTC5797 : Adjust pH of complete medium to 6.5.