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Distribution
조성 | 용량 | 단위 |
---|---|---|
KH2PO4 | 0.5 | g |
MgSO4·7H2O | 2.0 | g |
NaCl | 20.0 | g |
NH4Cl | 0.6 | g |
CaCl2·2H2O | 0.15 | g |
Sodium pyruvate | 3.0 | g |
Yeast extract | 0.3 | g |
Ferric Citrate solution(0.1% [w/v])(see medium 935) | 5.0 | ml |
Na2S·9H2O | 0.24 | g |
Micronutrient solution SL7(see medium 935) | 1.0 | ml |
Final pH 7/0+-0.2 | ||
Distilled water | 1.0 | L |
Mix ingredients except for NaHCO3 and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize NaHCO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add them. | ||
NaHCO3 (10% (w/v)) | 10.0 | ml |
Resazurin | 1.0 | mg |
Peptone | 0.3 | g |
** ph : -
** memo : -
** 추가조치 사항 : -