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미생물클러스터
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MICROBIOTA
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회원가입
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ENG
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Korean Collection for Type Cultures
KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
분양
일반자원검색
KCTC Media No. 991 : CLOSTRIDIUM THERMOCELLUM MEDIUM
| 조성 | 용량 | 단위 |
|---|---|---|
| (NH4)2SO4 | 1.30 | g |
| MgCl2·6 H2O | 2.60 | g |
| KH2PO4 | 1.43 | g |
| K2HPO4 | 5.50 | g |
| CaCl2· 2 H2O | 0.13 | g |
| Na2-ß-glycerol phosphate· 5 H2O | 6.00 | g |
| FeSO4· 7 H2O solution (0.1% w/v in 0.1 N H2SO4) | 1.10 | ml |
| L-Glutathione reduced | 0.25 | g |
| Yeast extract | 4.50 | g |
| Na-resazurin solution (0.1% w/v) | 0.5 | ml |
| Distilled water | 1000 | ml |
| Dissolve ingredients except cellobiose, sparge medium with 80% N2 and 20% CO2 gas mixture for 30 ? 45 min to make it anoxic. Then adjust pH to 7.0 - 7.2, distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Cellobiose is added to the sterile medium from an anoxic 10% (w/v) stock solution prepared under 100% N2 gas and sterilized by filtration. Some strains can be adapted to cellulose as substrate using 10.00 g/l cellulose (Avicel or MN 301, MACHEREY-NAGEL). Note: A white precipitate forms after mixing the ingredients of this medium, but this has no negative effect on growth. | ||
| Cellobiose | 5.00 | g |
** ph : -
** memo : DSM 122 medium
** 추가조치 사항 : -
담당자
기탁/분양서류
DNA분양서비스
동결건조서비스
STR분석서비스