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Media Information
Media No. 1010 EBA MEDIUM
| Composition | capacity | unit |
|---|---|---|
| CaCl2 ·2 H2O | 0.15 | g |
| MgSO4 ·7 H2O | 0.50 | g |
| KH2PO4 | 0.20 | g |
| Biotin (10 mg/l) (autoclave for 10 minutes under N2) | 0.20 | ml |
| Glycine | 2.0 | g |
| Trace element sol. SL-10 (see medium 497) | 1.0 | ml |
| NH4Cl | 0.25 | g |
| NaCl | 1.0 | g |
| Na2SeO3 ·5 H2O (0.26 g/ 1000 ml 10 mM NaOH) | 1.0 | ml |
| Resazurin | 1.00 | mg |
| Distilled water | 950.0 | ml |
| KCl | 0.50 | g |
| Before inoculation add per 100 ml of medium: | ||
| Biotin (10 mg/l)(autoclave for 10 minutes under N2) | 0.2 | ml |
| Na2S (60 g/l) (autoclaved under N2) | 1.0 | ml |
| pH 7.2 to 7.4 | ||
| NaHCO3 (84 g/l) (autoclaved under CO2) | 3.0 | ml |
** ph : -
** memo : -
** additional actions :