JOIN US
KOR
Korean Collection for Type Cultures
KCTC provides the distribution services
Find Resources
Media Information
Media No. 1033 Alkaliphilic spirochaete medium (annexure-1)
| Composition | capacity | unit |
|---|---|---|
| NaHCO3 | 15.0 | g |
| Na2CO3 | 10.0 | g |
| NaCl | 10.0 | g |
| Sucrose | 5.0 | g |
| K2HPO4 | 0.2 | g |
| NH4Cl | 1.0 | g |
| Trace element solution (see medium 334) | 1.0 | ml |
| Na2S · 9 H2O | 1.0 | g |
| KCl | 0.2 | g |
| Vitamin solution (see medium 334) | 10.0 | ml |
| Yeast extract | 0.5 | g |
| Distilled water | 1000 | ml |
| Prepare the medium under N2, Omitting NaHCO3, Na2S, sucrose and vitamin solution. Boil the medium and cool under nireogen, add the NaHCO3 and adjust the pH to 9.7 with 6N NaOH(about 15ml). Sterilize by autoclaving and add the Na2S(neutralized), vitamins and sucrose from sterile stock solutions. |
** ph : -
** memo : -
** additional actions :