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Media No. 1033 Alkaliphilic spirochaete medium (annexure-1)
| Composition | capacity | unit |
|---|---|---|
| NaHCO3 | 15.0 | g |
| Sucrose | 5.0 | g |
| Distilled water | 1000 | ml |
| NaCl | 10.0 | g |
| K2HPO4 | 0.2 | g |
| Vitamin solution (see medium 334) | 10.0 | ml |
| Prepare the medium under N2, Omitting NaHCO3, Na2S, sucrose and vitamin solution. Boil the medium and cool under nireogen, add the NaHCO3 and adjust the pH to 9.7 with 6N NaOH(about 15ml). Sterilize by autoclaving and add the Na2S(neutralized), vitamins and sucrose from sterile stock solutions. | ||
| NH4Cl | 1.0 | g |
| Na2S · 9 H2O | 1.0 | g |
| Trace element solution (see medium 334) | 1.0 | ml |
| KCl | 0.2 | g |
| Yeast extract | 0.5 | g |
| Na2CO3 | 10.0 | g |
** ph : -
** memo : -
** additional actions :