Korean Collection for Type Cultures

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Media No. 1041 MMB MEDIUM

Composition

capacity

unit

Peptone

0.15

Yeast extract

0.15

Hutner's salts

20.00

Composition	capacity	unit
Nitrilotriacetic acid (NTA)	10.000	g
MgSO4 x 7 H2O	29.700	g
CaCl2 x 2 H2O	3.335	g
(NH4)6MoO7O24 x 4 H2O	9.250	mg
FeSO4 x 7 H2O	99.000	mg
"Metals 44"	50.000	ml
Distilled water	950.000	ml
ZnSO4 x 7 H2O	1095.000	mg
FeSO4 x 7 H2O	500.000	mg

Vitamin solution

10.00

Composition	capacity	unit
Vitamin B12	0.01	mg
Biotin	0.20	mg
Ca-pantothenate	0.50	mg
Folic acid	0.20	mg
Pyridoxine-HCl	0.50	mg
Nicotinamide	0.50	mg
Riboflavin	0.50	mg
Thiamine-HCl x 2 H2O	0.50	mg
Distilled water	100.00	ml

(NH4)2SO4

0.25

Distilled water

970.00

Glucose

1.00

"Metals 44"

Composition	capacity	unit
Na-EDTA	250.000	mg
ZnSO4 x 7 H2O	1095.000	mg
FeSO4 x 7 H2O	500.000	mg
MnSO4 x H2O	154.000	mg
CuSO4 x 5 H2O	39.200	mg
Co(NO3)2 x 6 H2O	24.800	mg
Na2B4O7 x 10 H2O	17.700	mg
Distilled water	1000.000	ml

** ph : 7.2

** memo : Glucose and vitamin solution should be filter-sterilized separately.
Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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