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Media Information

Media No. 1052 Magntospirillum medium

Composition

capacity

unit

KH2PO4

0.68

Na-thioglycolate

0.05

Fe(III) quinate Solution, 0.01 M

2.0

Composition	capacity	unit
FeCl3 x 6 H2O	0.45	g
Quinic acid	0.19	g
Distilled water	100	ml

Trace elements

5.0

Composition	capacity	unit
Nitrilotriacetic acid	1.5	g
MgSO4 x 7 H2O	3.0	g
MnSO4 x H2O	0.5	g
NaCl	1.0	g
FeSO4 x 7 H2O	0.1	g
CoSO4 x 7 H2O	0.18	g
CaCl2 x 2 H2O	0.1	g
ZnSO4 x 7 H2O	0.18	g
CuSO4 x 5 H2O	0.01	g
KAl(SO4)2 x 12 H2O	0.02	g
H3BO3	0.01	g
Na2MoO4 x 2 H2O	0.01	g
NiCl2 x 6 H2O	0.03	g
Na2SeO3 x 5 H2O	0.3	g
Distilled water	1.0	L
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

L(+)-Tartaric acid

0.37

NaNO3

0.12

Vitamin solution

10.0

Composition	capacity	unit
Biotin	2.0	mg
Folic acid	2.0	mg
Pyridoxine-HCl	10.0	mg
Thiamine-HCl x 2 H2O	5.0	mg
Riboflavin	5.0	mg
Nicotinic acid	5.0	mg
D-Ca-pantothenate	5.0	mg
Vitamin B12	0.1	mg
p-Aminobenzoic acid	5.0	mg
Lipoic acid	5.0	mg
Distilled water	1.0	L

Na-acetate

0.05

Distilled water

1.0

Resazurin

0.5

Succinic acid

0.37

Agar (for semi-solid agar)

1.30

** ph : 6.75

** memo : Ferric Quinate was replaced by Ferric Citrate(0.01M)

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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