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Media Information
Media No. 1169 MH1
| Composition | capacity | unit |
|---|---|---|
| Yeast extract (BD-Difco) | 2.0 | g |
| L-Glutamic acid | 2.0 | g |
| Trisodium citrate·2H2O | 2.0 | g |
| KH2PO4 | 1.0 | g |
| NH4Cl | 1.0 | g |
| NaCl | 200 | g |
| K2SO4 | 5.0 | g |
| Casamino acids (BD-Difco) | 4 | g |
| MgCl2·6H2O | 20.0 | g |
| 0.2% FeSO4·6H2O solution | 2.0 | ml |
| Trace metal solution(배지번호1686) | 2.0 | ml |
** ph : 5.2
** memo : For preparation of solid medium, add the components listed above (reduce the amount of NaCl to 150.0 g) to distilled water and bring volume to 800.0 ml. Separately prepare the following solution:
NaCl 50.0 g
Agar 20.0 g
Distilled water 200.0 ml
Combine both solutions after autoclaving.
** additional actions :