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Solution B | 500 | ml | |||||||||||||||||||||||||||||||||
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Solution A | 500 | ml | |||||||||||||||||||||||||||||||||
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Trace element solution | |||||||||||||||||||||||||||||||||||
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** ph : 4.5
** memo : ●Solution A 500ml
●Solution B 500ml
Agar solid medium of GYS contained 2% agar.
In order to prevent the hydrolysis of agar and glucose by autoclaving at low pH condition, Solution A and B are autoclaved separately and combined before solidified(50-60℃)
Adjust pH WITH 1N H_2_SO_4_(pH4.5)
** additional actions :