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Media Information
Media No. 1200 RCM(Reinforced Clostridial Medium)
| Composition | capacity | unit |
|---|---|---|
| Peptone | 10.0 | g |
| Starch | 10.0 | g |
| Beef extract | 10.0 | g |
| Yeast extract | 3.0 | g |
| Na2S(filteration sterilization) | 0.5 | g |
| Glucose | 5.0 | g |
| NaCl | 3.0 | g |
| Cysteine | 0.5 | g |
| Sodium acetate | 3.0 | g |
| Cystein·HCl | 0.5 | g |
| D.W | 1000 | ml |
| Resazurin | 0.001 | g |
** ph : 7.0
** memo : Attention: Na2S was filter-sterilized and added after the medium be autoclaved, we usually poured the medium in anaerobic workstation, and the strain be incubated in the workstation, too.
** additional actions :