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Media No. 1247 SSE/HD 1:10 Medium

Composition

capacity

unit

MES(10mM final concentration)

1.95

Distilled water

500

Glucose

0.1

Peptone

0.5

Yeast extract

0.25

Vitamin solution

1.0

Composition	capacity	unit
Biotin	2.0	mg
Folic acid	2.0	mg
Pyridoxine-HCl	10.0	mg
Thiamin-HCl·2H₂O	5.0	mg
Riboflavin	5.0	mg
Nicotinic acid	5.0	mg
D-Ca-pantothenate	5.0	mg
Vitamin B_12	0.1	mg
p-Aminobenzoic acid	5.0	mg
Lipoic acid	5.0	mg
Distilled water	1000	ml

Add to 1000ml of medium after autoclaving:

adjust pH to 5.5

SSE(double concentrated)(see below)

500

Composition	capacity	unit
CaCl_2·2H₂O	0.2938	g
NH₄Cl	0.1069	g
MgCl_2·6H₂O	0.2036	g
(NH_4)₂SO_4	0.1983	g
MgSO_4·7H₂O	0.7390	g
CaSO_4·2H₂O	0.8606	g
Ca(NO_3)_2·4H₂O	0.2360	ml
NaNO_3	0.4240	g
KH₂PO_4 (100mM solution)	0.5000	ml
FeSO_4·7H₂O	0.0111	g
K₂SO_4	0.0870	g
add distilled water	1000	ml
Dissolve by shaking overnight. Sterilize by autoclaving.

Trace Element Solution SL-10

1.0

Composition	capacity	unit
HCl (25%; 7.7M)	10.0	ml
FeCl_2·4H₂O	1.5	g
ZnCl_2	70	mg
MnCl_2·4H₂O	100	mg
H₃BO_3	6	mg
CoCl_2·6H₂O	190	mg
CuCl_2·2H₂O	2.0	mg
NiCl_2·6H₂O	24	mg
Na₂MoO_4·2H₂O	36	mg
Distilled water	990	ml
First dissolve FeCl_2 in HCl, then dilute with water, add and dissolve the other salts. Finally make up to 1000.0ml.

** ph : 5.5 / 7

** memo : Medium for KCTC52215 (PH7)
Medium for KCTC52626 (PH5.5)

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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