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Media No. 1247 SSE/HD 1:10 Medium

Composition

capacity

unit

Glucose

0.1

Peptone

0.5

Yeast extract

0.25

adjust pH to 5.5

SSE(double concentrated)(see below)

500

Composition	capacity	unit
CaCl_2·2H₂O	0.2938	g
NH₄Cl	0.1069	g
MgCl_2·6H₂O	0.2036	g
(NH_4)₂SO_4	0.1983	g
MgSO_4·7H₂O	0.7390	g
CaSO_4·2H₂O	0.8606	g
Ca(NO_3)_2·4H₂O	0.2360	ml
NaNO_3	0.4240	g
KH₂PO_4 (100mM solution)	0.5000	ml
FeSO_4·7H₂O	0.0111	g
K₂SO_4	0.0870	g
add distilled water	1000	ml
Dissolve by shaking overnight. Sterilize by autoclaving.

MES(10mM final concentration)

1.95

Vitamin solution

1.0

Composition	capacity	unit
Biotin	2.0	mg
Folic acid	2.0	mg
Pyridoxine-HCl	10.0	mg
Thiamin-HCl·2H₂O	5.0	mg
Riboflavin	5.0	mg
Nicotinic acid	5.0	mg
D-Ca-pantothenate	5.0	mg
Vitamin B_12	0.1	mg
p-Aminobenzoic acid	5.0	mg
Lipoic acid	5.0	mg
Distilled water	1000	ml

Add to 1000ml of medium after autoclaving:

Distilled water

500

Trace Element Solution SL-10

1.0

Composition	capacity	unit
HCl (25%; 7.7M)	10.0	ml
FeCl_2·4H₂O	1.5	g
ZnCl_2	70	mg
MnCl_2·4H₂O	100	mg
H₃BO_3	6	mg
CoCl_2·6H₂O	190	mg
CuCl_2·2H₂O	2.0	mg
NiCl_2·6H₂O	24	mg
Na₂MoO_4·2H₂O	36	mg
Distilled water	990	ml
First dissolve FeCl_2 in HCl, then dilute with water, add and dissolve the other salts. Finally make up to 1000.0ml.

** ph : 5.5 / 7

** memo : Medium for KCTC52215 (PH7)
Medium for KCTC52626 (PH5.5)

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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