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Media No. 1283 Fresh Water Medium for soil AOA

Composition

capacity

unit

NaCl

1.0

KH₂·PO₄

0.2

Basic FWM solution

MgCl₂6H₂O

0.4

Vitamin solution

1.0

Composition	capacity	unit
Biotin	20.0	mg
Folic acid	20.0	mg
Pyridoxine-HCl	100	mg
Thiamine-HCl	50	mg
Riboflavin	50	mg
Nicotinic acid	50	mg
DL-Calcium pantothenate	50	mg
Vitamine B₁₂	1.0	mg
p-Aminobenzoic acid	50	mg
Choline chloride	2.0	g
D.W	1.0	L
Adjust pH to 7.0 with KOH.

CaCl₂·2H₂O

0.1

Modified trace element mixture

1.0

Composition	capacity	unit
HCl (25%)	12.5	ml
H₃BO₃	30.0	mg
MnCl₂·4H₂O	100	mg
CoCl₂·6H₂O	190	mg
NiCl₂·6H₂O	24	mg
CuCl₂·2H₂O	2.0	mg
ZnSO₄·7H₂O	144	mg
Na₂MoO₄·2H₂O	36	mg
FeCl₂·4H₂O	1.49	g
Na₂·EDTA	5.2	g
D.W	987	ml

HEPES solution

5.0

Composition	capacity	unit
NaOH	12	g
HEPES(free acid)	119.2	g

MilliQ water

1.0

KCl

0.5

1M Sodium pyruvate solution

1.0

Autoclave the basic FWM solution and aspectically add the following solutions(autoclaved or filter-sterilized) in the given order:

1M NaHCO₃ solution

2.0

1M NH₄Cl solution

1.0

Finally, adjust pH to 7.0 with NaOH or HCl.

** ph : 7

** memo : for KCTC4249

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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