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Media Information
Media No. 134 Bacillus Medium No. 1
| Composition | capacity | unit |
|---|---|---|
| (NH4)2SO4 | 1.3 | g |
| KH2PO_4 | 0.37 | g |
| MgSO4·7H2O | 0.25 | g |
| CaCl2·2H2O | 0.07 | g |
| FeCl3 | 0.02 | g |
| Glucose | 1.0 | g |
| Yeast extract | 1.0 | g |
| Distilled water | 1.0 | L |
** ph : 4.0
** memo : Adjust pH to 4.0 with 10N H_2_SO_4_. For solid medium, make up above solution in double strength and adjust pH to 3.5. Make water agar at 40 g/L. Autoclave separately. Cool to about 50°C and mix equal volumes of both solutions aseptically. This procedure avoids acid hydrolysis of the agar.
** additional actions :