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Media No. 1344 Sedimentisphaera(L21) Medium
| Composition | capacity | unit |
|---|---|---|
| MgCl2·6H2O | 6 | g |
| Na2SO4 | 1.0 | g |
| KCl | 1.5 | g |
| NaCl | 120 | g |
| Trace element solution(see medium 918) | 10.0 | ml |
| CaCl2·2H2O | 0.4 | g |
| Na-resazurin solution(0.1% w/v) | 0.5 | ml |
| L-Cysteine-HCl·H2O | 0.5 | g |
| Vitamin solution(see medium 1483) | 1.0 | ml |
| NH4Cl | 1.0 | g |
| Dissolve ingredients except carbonate, glucose, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.5, if necessary. Note: Use at least 5-10%(v/v) inoculum. | ||
| Na2S·9H2O | 0.5 | g |
| D-Glucose | 1.0 | g |
| K2HPO4 | 0.4 | g |
| Na2CO3 | 2.5 | g |
| D.W | 1.0 | L |
** ph : 7.5
** memo : -
** additional actions :