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Media Information

Media No. 1374 Modified ANNEXURE-I medium.

Composition

capacity

unit

NaHCO₃ solution(10%(w/v))

15.0

ml/L

Composition	capacity	unit
NaHCO₃(10g) was dissolved in distilled water(100ml) in 250ml screw capped bottle and bubbled with CO₂ for 5 minutes and the bottle was closed tightly under CO₂ atmosphere and autoclaved for 15 minutes at 15 lbs and used for media preparation.

CaCl₂·2H₂O

0.15

g/L

NH₄Cl

0.68

g/L

MgSO₄·7H₂O

2.0

g/L

Na₂S·9H₂O (1M)

1.0

ml/L

Composition	capacity	unit
Na₂S·9H₂O (2.4g) was dissolved in distilled water(10ml) in 15ml screw cap test tube and flushed with nitrogen gas for 2-3 minutes to replace the air in the tube with nitrogen gas and closed tightly and was autoclaved.

Ferric citrate solution(0.1%,w/v)

5.0

ml/L

KH₂PO₄

0.5

g/L

Pyruvate

3.0

g/L

NaCl

20.0

g/L

Yeast Extract

0.3

g/L

Micronutrient solution(SL7)

1.0

ml/L

Composition	capacity	unit
ZnCl₂	70	mg/L
MnCl₂·4H₂O	100	mg/L
H₃BO₃	60	mg/L
CoCl₂·6H₂O	200	mg/L
NiCl₂·6H₂O	20.0	mg/L
Na₂MoO_4·2H₂O	40	mg/L
CuCl₂·2H₂O	20.0	mg/L
HCl(25%v/v)	1.0	ml
pH was adjusted to 7.0-7.5 with 1N NaOH/1N HCl.

Vitamin B12 solution

1.0

ml/L

Composition	capacity	unit
Vitamin B12(2mg/1000ml) was dissolved in distilled water and filter sterilized by using 0.2um pore sized Millipore cellulose acetate membrane filter into a sterile screw cap tube.

** ph : 7.5

** memo : medium for KCTC 15634.

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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