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Composition | capacity | unit | ||||||||||||||||||||||||
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glucose | 5.0 | g/L | ||||||||||||||||||||||||
(NH4)2SO4 | 2.0 | g/L | ||||||||||||||||||||||||
MgSO4·7H2O | 0.5 | g/L | ||||||||||||||||||||||||
K2HPO4·3H2O | 0.5 | g/L | ||||||||||||||||||||||||
yeast extract | 0.25 | g/L | ||||||||||||||||||||||||
KCl | 0.1 | g/L | ||||||||||||||||||||||||
Trace solution(pH 2.0) | 1.0 | ml/L | ||||||||||||||||||||||||
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** ph : 4.0
** memo : For the preparation of solid plate, equal volumes of autoclaved, twice-concentrated G4 broth(pH adjusted to 4.0) and gelrite(11g/L, Sigma) solutions were mixed gently before the plates were made.
** additional actions :