Korean Collection for Type Cultures

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Media Information

Media No. 1427 YCFA Medium (Modified 6)

Composition

capacity

unit

yeast extract

4.0

g/L

Cysteine-HCl

0.5

g/L

CaCl₂·2H₂O

0.11

g/L

soy peptone

2.0

g/L

Haemin solution

1.0

Composition	capacity	unit
Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water. Store refrigerated.

vitamin solution

Composition	capacity	unit
biotin	0.02	g/L
niacin	0.2	g/L
pyridoxine	0.5	g/L
riboflavin	0.1	g/L
thiamine	0.2	g/L
cyanocobalamin	0.1	g/L
p-aminobenzoic acid	0.1	g/L
pantothenic acid	0.1	g/L

KH₂PO₄

0.41

g/L

MgCl₂·6H₂O

0.1

g/L

casitone

2.0

g/L

NaHCO₃

g/L

trace elements II (acid)

Composition	capacity	unit
FeCl₂	7.5	mM
H₃BO₄	1.0	mM
ZnCl₂	0.5	mM
CuCl₂	0.1	mM
MnCl₂	0.5	mM
CoCl2	0.5	mM
NiCl₂	0.1	mM
HCl	50	mM

Vitamin K1 solution

0.2

Composition	capacity	unit
Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

trace elements I (alkaline)

Composition	capacity	unit
Na₂SeO₃	0.1	mM
Na₂WO₄	0.1	mM
Na₂MoO₄	0.1	mM
NaOH	10.0	mM

** ph : 7.0

** memo : The medium was supplemented with 30mM of sodium acetate. For routine use, the medium was distributed in 35 ml serum bottles sealed with butyl-rubber stoppers at 37 °C under a gas phase of 1.7 atm (172 kPa) N2/CO2 (80 : 20, v/v). The pH of the medium was 7.0.

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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