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Media Information
Media No. 1481 Strictly anaerobic modified freshwater Pfennig basal medium
| Composition | capacity | unit |
|---|---|---|
| KH2PO4 | 0.33 | g/L |
| KCl | 0.33 | g/L |
| MgCl2 | 0.33 | g/L |
| CaCl2·2H2O | 0.33 | g/L |
| Trace Element Solution (Kevbrin and Zavarzin, 1992) (see the medium No.1482) | 1.0 | ml/L |
| Na2S | 0.3 | g/L |
| NH4Cl | 0.33 | g/L |
| Yeast extract | 200 | mg/L |
| Vitamin Solution (Wolin et al., 1963) (See KCTC medium No.1335) | 1.0 | ml/L |
** ph : 6.0-6.5
** memo : Prepare medium under N2
After sterilization add Sucrose 1 g/l and Th.aggregans culture broth filtrate 10ml/l.
** additional actions :