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Media Information

Media No. 1502 Modified M-30 Media

Composition

capacity

unit

MgCl₂

1.0

g/L

Important: Dissolve the nitrilotriaceticacid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H₂SO₄.

(filter-sterilize)

Artificial sea water

955

Composition	capacity	unit
NaCl	23.47	g
Na₂SO₄	3.92	g
MgCl₂·6H₂O	4.98	g
CaCl₂	1.10	g
NaHCO₃	192	mg
KCl	664	mg
KBr	96	mg
H₃BO₃	26	mg
SrCl₂	24	mg
NaF	3.0	mg
Distilled water	1000	ml

Hutner basal salt

20.0

Composition	capacity	unit
Nitrilotriacetic acid (NTA)	10.0	g
MgSO₄·7H₂O	29.7	g
CaCl₂·2H₂O	3.335	g
FeSO₄·7H₂O	99	mg
(NH₄)6MoO_7·4H₂O	9.250	mg
Metals 44 (see below)	50	ml
Distilled water	950	ml

N-acetylglucosamine (Sigma)

2.0

Vitamin solution (See below)

10.0

Composition	capacity	unit
Vitamin B₁₂	0.2	mg
Thiamine-HCl ·2H₂O	10.0	mg
Folic acid	4	mg
Ca-Pantothenate	10.0	mg
Biotin	4	mg
Riboflavin	10.0	mg
Nicotinamide	10.0	mg
p-Aminobenzoic acid	10.0	mg
Pyridoxine hydrochloride	10.0	mg
Distilled water	1000	ml

autoclave solution 1 at 121?C for 15 min.

1 M Tris-HCl (pH 8)

5.0

Na₂HPO₄·2H₂O

0.01

Agar

20.0

g/L

FeSO₄·7H₂O

500

Filter sterilize solution 2, and add to solution 1. Important: Adjust pH of media to 7.5 to 8,

Distilled water

10.0

Na-EDTA

250

ZnSO₄·7H₂O

1095

Co(NO₃)₂·6H₂O

24.8

MnSO₄·H₂O

154

Na₂B₄O₇·10H₂O

17.7

CuSO₄·5H₂O

39.2

Distilled water

1000

Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.Filter sterilize

** ph : 7.8

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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