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Composition | capacity | unit |
---|---|---|
NH4Cl | 0.25 | g/L |
KCl | 0.36 | g/L |
MgCl2·6H2O | 0.4 | g/L |
CaCl2 | 0.05 | g/L |
KH2PO4 | 0.14 | g/L |
Trace Element Solution (Kevbrin and Zavarzin, 1992) (see the medium No.1482) | 1.0 | ml/L |
Vitamin Solution (Wolin et al., 1963) (see the medium No.1483) | 1.0 | ml/L |
HEPES | 10.0 | mM |
** ph : 7.2-7.5
** memo : pH was adjusted with 6M HCl or 10M NaOH. Autoclaving was carried out at 120℃ and 1 AT during an hour. After sterilizing, substrate - yeast extract, or peptonem or acetate(0.5g/L) - was added.
** additional actions :