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Media No. 1542 NAS-02 MEDIUM
| Composition | capacity | unit |
|---|---|---|
| (NH4)2SO4 | 0.2 | g/L |
| 5% (w/v) MgSO4·7H2O solution | 10.0 | ml |
| KH2PO4 | 3.0 | g/L |
| Mix components thoroughly and adjust pH to 5.2. Autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions anaerobically and aseptically (autoclaved or ·filter-sterilized and store under a N2 atmosphere): | ||
| Na2S2O3·5H2O | 2.5 | g/L |
| 25 mM L-Cysteine·HCl solution | 10.0 | ml |
| Modified Allen's trace metal solution(see Medium No. 1543) | 1.0 | ml/L |
| 5% (w/v) CaCl2·2H2O solution | 5.0 | ml |
| 130 mM FeCl2 solution | 10.0 | ml |
| Distilled water | 1.0 | L |
| 10% (w/v) Yeast extract solution | 5.0 | ml |
| Trace vitamins· (see Medium No. 1544) | 10.0 | ml |
** ph : -
** memo : -
** additional actions :