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Media No. 1550 Acid mine drainage solid medium

Composition

capacity

unit

Yeast extract

0.4

g/L

2X liquid medium (pH 2.7) mix with 1% Gelrite equally after sterilization

Composition	capacity	unit
(NH_4)_2SO4	3.0	g/L
MgSO_4·7H_2O	0.5	g/L
Na_2SO4·10H_2O	0.15	g/L
KH_2PO4	0.1	g/L
KCI	0.1	g/L
Ca(NO_3)_2·4H_2O	0.014	g/L
(Trace elements solution)	2000X
-> ZnSO_4·7H_2O	10.0	g/L
-> CuSO_4·5H_2O	1.0	g/L
-> Cr_2(SO_4)_3·15H_2O	0.5	g/L
-> Na_2B4O_7·10H_2O	0.5	g/L
-> CoSO_4·7H_2O	1.0	g/L
-> MnSO_4·4H_2O	1.0	g/L
-> Na_2MoO4·2H_2O	0.5	g/L
-> NaVO_3	0.1	g/L
-> pH 2.0 (with 3M H_2SO4)

Glucose

0.4

g/L

FeSO_4·7H_2O

0.05

** ph : 2.5-3.5

** memo : 1% Gelrite로는 배지가 굳지 않음. % 더 높일 것.

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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