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| Composition | capacity | unit |
|---|---|---|
| Yeast extract | 5.0 | g |
| Glucose | 50.0 | g |
| CaCO3 | 12.5 | g |
| Distilled water | 1.0 | L |
| Agar | 15.0 | g |
** ph : -
** memo : Autoclave at 10 lbs. for 30 min. Place tube in 48℃ water bath. Resuspend calcium carbonate by vibrating individual tubes and slant.
** additional actions :