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Media No. 1663 ANAEROBIOSPIRILLUM THOMASII MEDIUM
| Composition | capacity | unit |
|---|---|---|
| 5% (w/v) NaHCO_3 (flushed with N_2 +CO_2) | 0.5 | ml |
| 3% (w/v) Na_2S · 9H_2O (under N_2) | 0.1 | ml |
| 3% (w/v) cysteine-HCl · 2H_2O (under N_2) | 0.1 | ml |
| Fildes Enrichment (BBL 20810) (under N_2) | 1.0 | ml |
** ph : 6.8-7.0
** memo : Use Wilkins Chalgrene Anaerobe Broth (Oxoid CM 643) and prepare it anaerobically under 80% N_2 + 20% CO_2 gas. Agter autoclaving and cooling to room temperature add per 10ml the following sterile, anaerobic solutions :
** additional actions :