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Media No. 1680 ANAEROBIC NATURAL SEAWATER MEDIUM
| Composition | capacity | unit |
|---|---|---|
| Resazurin | 0.5 | mg |
| Yeast extract | 1.0 | g |
| Tryptone | 5.0 | g |
| Natural seawater (filtrated) | 750 | ml |
| 5% Na_2S·9H_2O solution | 6 | ml |
| Distilled water | 250 | ml |
| 5% L-Cysteine·HCl·H_2O solution | 6 | ml |
| Mix components thoroughly and adjust pH to 7.0. Bring to a boil and cool down under a N2 gas atmosphere. Distribute the medium in culture vessels (e.g., 10 ml in Balch tubes) under N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere): |
** ph : -
** memo : Mix components thoroughly and adjust pH to 7.0. Bring to a boil and cool down under a N2 gas atmosphere. Distribute the medium in culture vessels (e.g., 10 ml in Balch tubes) under N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
** additional actions :