JOIN US
KOR
Korean Collection for Type Cultures
KCTC provides the distribution services
Find Resources
Media Information
Media No. 1682 A1 Medium
| Composition | capacity | unit | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KCl | 0.77 | g | ||||||||||||
| NH4Cl | 0.25 | g | ||||||||||||
| NaCl | 20.0 | g | ||||||||||||
| KH2PO4 | 0.1 | g | ||||||||||||
| Vitamin solution (see medium 1335) | 10.0 | ml | ||||||||||||
| Trace element solution (see medium 918) | 10.0 | ml | ||||||||||||
| MgSO4·7H2O | 0.2 | g | ||||||||||||
| Mix ingredients except NaHCO3, adjust pH to 6.8 and add NaHCO3 to the medium. Dispense the medium (9 ml) into 26-ml anaerobic pressure tubes under a stream of oxygen-free N2-CO2 (80:20) and seal. After autoclaving, aseptically and anaerobically add 0.2 ml APM salts solution and 0.1 ml Sulfide stock solution and 0.1 ml Galactose stock solution. | ||||||||||||||
| APM salts solution (autoclaved) | 0.2 | ml | ||||||||||||
|
||||||||||||||
| NaHCO3 | 2.0 | g | ||||||||||||
| Galactose stock solution (filter-sterilized) | 0.1 | ml | ||||||||||||
|
||||||||||||||
| Sulfide stock solution (autoclaved) | 0.1 | ml | ||||||||||||
|
||||||||||||||
| D.W | 1.0 | L | ||||||||||||
** ph : -
** memo : -
** additional actions :