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Media Information
Media No. 1749 Anaerobic modified Widdel medium
| Composition | capacity | unit |
|---|---|---|
| KH2PO4 | 0.2 | g/L |
| NH_4Cl | 0.25 | g/L |
| MgCl_2·6H_2O | 0.4 | g/L |
| CaCl2·6H2O | 0.1 | g/L |
| KCl | 0.5 | g/L |
| Vitamin Solution (Wolin et al., 1963) | 1.0 | ml/L |
| NaCl | 1.0 | g/L |
| Substrate -pyruvate | 10.0 | mM |
| Trace element solution (Kevbrin, Zavarzin, 1992) | 1.0 | ml/L |
| Yeast extract | 0.5 | g/L |
| pH was adjusted with 6M HCl or 10M NaOH. Autoclaving was carried out at 120°C and 1 ATM during an hour. |
** ph : 7
** memo : -
** additional actions :