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Media Information

Media No. 1772 M8

Composition

capacity

unit

peptone

0.5

g/L

KCl

0.72

g/L

sodium acetate

2.0

g/L

NaCl

22.79

Na_2SO4

3.98

g/L

glucose

0.2

g/L

sodium malate

0.05

g/L

yeast extract

0.5

g/L

potato extract powder

0.5

g/L

TAPSO

1.30

g/L

trisodium citrate

0.05

g/L

sodium potassium tartrate

0.05

g/L

solution I

2.0

ml/L

Composition	capacity	unit
NH_4Cl	135	g/L
Na_2HPO4·7H_2O	44.5	g/L
NaBr	41.5	g/L
NaHCO_3	15.5	g/L
H_2BO3	13.5	g/L
NaF	1.3	g/L

When using, add solution II (20ml per 1L) and solution III (1ml per lL)

adjusted pH 7.6-7.8, followed by steam (115 ℃, 15 min) sterilization

Solution II

20.0

ml/L

Composition	capacity	unit
SrCl_2·6H_2O	1.2	g/L
MgCl_2·6H_2O	559	g/L
CaCl_2·2H_2O	73	g/L

Solution III

1.0

ml/L

Composition	capacity	unit
FeSO_4·2H_2O	2.8	g/L

** ph : 7.6

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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