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Media No. 1782 MODIFIED NITRATE MINERAL SALTS MEDIUM-2
| Composition | capacity | unit | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 M HEPES solution (pH 7.0) | 2.0 | ml | |||||||||||||||
| Ammonium ferric citrate | 4 | mg | |||||||||||||||
| KNO_3 | 0.25 | g | |||||||||||||||
| Mix components thoroughly and autoclave. After cooling, add the following solutions (autoclaved) to the medium: | |||||||||||||||||
| FeCl_2 solution | 1.0 | ml | |||||||||||||||
|
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| MgSO_4·7H_2O | 1.0 | g | |||||||||||||||
| CaCl_2·2H_2O | 0.2 | g | |||||||||||||||
| Trace element solution (SL-10, KCTC Medium No.1417) | 1.0 | ml | |||||||||||||||
| Distilled water | 1.0 | L | |||||||||||||||
| Phosphate buffer solution | 2.0 | ml | |||||||||||||||
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| Vitamin solution | 10.0 | ml | |||||||||||||||
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** ph : 6.8
** memo : -
** additional actions :