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Media Information
Media No. 1807 Medium for strain JA349 and JA350
| Composition | capacity | unit |
|---|---|---|
| Na2S·9H2O | 0.24 | g |
| NaCl | 20.0 | g |
| Resazurin | 1.0 | mg |
| KH2PO4 | 0.5 | g |
| NH4Cl | 0.68 | g |
| Micronutrient solution SL7(see medium 935) | 1.0 | ml |
| MgSO4·7H2O | 3.0 | g |
| Mix ingredients except for vitamin B12, NaHCO3 and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize vitamin B12 and NaHCO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add them. | ||
| NaHCO3 (10% (w/v)) | 15.0 | ml |
| Yeast extract | 0.5 | g |
| Vitamin B12 (from 2mg/100ml)(see medium 935) | 1.0 | ml |
| Sodium pyruvate | 3.0 | g |
| Ferric Citrate solution(0.1% [w/v])(see medium 935) | 5.0 | ml |
| Distilled water | 1.0 | L |
| CaCl2·2H2O | 0.15 | g |
** ph : -
** memo : -
** additional actions :