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Composition | capacity | unit |
---|---|---|
MgSO4·7H2O | 3.0 | g |
KH2PO4 | 0.5 | g |
Na2S·9H2O | 0.24 | g |
CaCl2·2H2O | 0.15 | g |
Resazurin | 1.0 | mg |
NaCl | 20.0 | g |
Distilled water | 1.0 | L |
Yeast extract | 0.5 | g |
NH4Cl | 0.68 | g |
Micronutrient solution SL7(see medium 935) | 1.0 | ml |
Sodium pyruvate | 3.0 | g |
NaHCO3 (10% (w/v)) | 15.0 | ml |
Mix ingredients except for vitamin B12, NaHCO3 and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize vitamin B12 and NaHCO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add them. | ||
Ferric Citrate solution(0.1% [w/v])(see medium 935) | 5.0 | ml |
Vitamin B12 (from 2mg/100ml)(see medium 935) | 1.0 | ml |
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** memo : -
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