Korean Collection for Type Cultures

KCTC provides the distribution services

Find Resources

Media Information

Media No. 270 Minimal Salts (MS) Medium with colloidal chitin

Composition

capacity

unit

Solution A

ml/L

Composition	capacity	unit
KH₂PO₄	8.0	g/L
MgCl_2·6H₂O	4.0	g/L
NH₄Cl	5.0	g/L
CaCl₂·2H₂O	1.0	g/L

NaHCO₃

3.52

g/L

Resazurin(0.1%)

1.0

ml/L

Solution B

ml/L

Composition	capacity	unit
K₂HPO₄	8.0	g/L

Trace metal solution

10.0

ml/L

Composition	capacity	unit
Trace metal solution (100X)
Nitrilotriacetic acid	12.8	g/L
FeSO₄·7H₂O	0.42	g/L
MnCl₂·4H₂O	0.1	g/L
CoCl₂·6H₂O	0.17	g/L
CuCl₂·2H₂O	0.02	g/L
ZnSO₄·7H₂O	0.21	g/L
Na₂MoO₄·2H₂O	0.01	g/L

Wolfe's vitamin solution

10.0

ml/L

Composition	capacity	unit
Wolfe's vitamin solution (100X)
Biotin	2.0	mg/L
folic acid	2.0	mg/L
pyridoxine HCl	10.0	mg/L
thiamine HCl	5.0	mg/L
riboflavin	5.0	mg/L
nicotinic acid	5.0	mg/L
calcium D-(+)pantothenate	5.0	mg/L
cyanocobalamine	0.1	mg/L
p-aminobenzoic acid	5.0	mg/L
thioctic acid	5.0	mg/L

Yeast extracrt

1.0

g/L

Polypeptone

10.0

g/L

Colloidal chitin

5.0

g/L

** ph : 7-7.2

** memo : Adjust it with NaOH Final concentration of ~0.5% colloidal chitin was mixed with MS medium
as sole carbon substrate to grow the strain.

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

Copyright 2019 KCTC. All rights reserved.