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Media Information
Media No. 280 Cellulomonas Fermentans Medium
| Composition | capacity | unit |
|---|---|---|
| (NH4)2SO4 | 1.3 | g |
| MgCl2·6H2O | 0.1 | g |
| K2HPO4 | 2.21 | g |
| KH2PO4 | 1.5 | g |
| FeSO4·7H2O | 1.25 | mg |
| (MN 300) NaHCO3 | 0.8 | g |
| CaCl2·2H2O | 0.02 | g |
| Yeast extract | 5.0 | g |
| Distilled water | 1.0 | L |
| Cellobiose or Cellulose | 5.0 | g |
| Resazurin | 1.0 | mg |
| Cysteine hydrochloride | 0.5 | g |
** ph : 7.4
** memo : Adjust pH to 7.4 with 8M NaOH. Prepare the medium anaerobically under 100 % nitrogen. Cellobiose, NaHCO_3_, and cysteine are sterilized separately by autoclaving under N_2_ For aerobic growth omit resazurin and cysteine.
** additional actions :