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Media Information

Media No. 301 CYE (Charcoal Yeast Extract) buffered medium

Composition

capacity

unit

ACES Buffer

10.0

Solution A

Composition	capacity	unit
Distilled water	10.0	ml
L-CysteineHCl	0.4	g
Filter Sterilize.

Solution B

Composition	capacity	unit
Fe-pyrophosphate	0.25	g
Distilled water	10.0	ml
Filter Sterilize.
Note: Keep soluble Ferric pyrophosphate dry and in the dark. Do not use if the chemical loses its green color and becomes brown or yellow. Do not heat over 60ºC to dissolve. The mixture dissolves readily in a 50ºC water bath.

Charcoal (Sigma C5510)·

2.0

Yeast extract (Difco 0127)

10.0

Agar

17.0

Adjust pH to 6.90 +/- 0.05 using KOH (pH is critical). Autoclave at 121ºC. Cool to 50ºC. Aseptically add Solutions A and B. Recheck pH and adjust if necessary.

Distilled water

980

** ph : -

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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