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Composition | capacity | unit |
---|---|---|
FeCl3·6H2O | 0.01 | g |
Yeast extract | 2.5 | g |
CaCO3 | 1.0 | g |
NaCl | 30.0 | g |
Agar (if required) | 15.0 | g |
MgSO4·7H2O | 0.3 | g |
Distilled deionized water | 1.0 | L |
KH2PO4 | 3.0 | g |
Sodium Glycerol Phosphate (Sigma G-6501) | 23.5 | g |
Tryptone | 5.0 | g |
NH4Cl | 0.3 | g |
** ph : -
** memo : Heat to dissolve completely. Boil if agar is included. Adjust pH to 7.0 +/- 0.2. Autoclave at 121C for 15 minutes.
** additional actions :