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Media No. 459 BG11

Composition

capacity

unit

· Trace metal mix A5

Composition	capacity	unit
Distilled Water	994	ml
MnCl₂ . 4H₂O	1.81	g
ZnSO₄ . 7H₂O	0.222	g
Na₂MoO₄ . 2H₂O	0.39	g
CuSO₄ . 5H₂O	0.079	g
Co(NO₃)₂ . 6H₂O	0.049	g
H₃BO₃	2.86	g

Preparation: to add 929 ml of glass-distilled water, add the following stock solutions in the quanitities indicated.

Stock solution ( g/ 400 ml H₂O)

Composition	capacity	unit
1 ml Trace metal mix A5
5 ml Feric ammonium citrate	0.48	g
5 ml EDTA	0.08	g
10 ml Citric acid	0.24	g
10 ml MgSO₄ . 7H₂O	3.0	g
10 ml CaCl₂ . 2H₂O	1.44	g
10 ml NaNo₃	60	g
10 ml K₂HPO₄	1.6	g
10 ml Na₂CO₃	0.8	g

** ph : -

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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