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Media Information

Media No. 476 Middlebrook 7H10 Agar

Composition

capacity

unit

Glycerol

5.0

Distilled water

900

Sterilize for 10 min by 121℃ cool to 50 - 55℃. Add 100 ml Bacto Middelbrook OADC enrichment Difco (see below) under aseptic conditions mix thoroughly.

Bacto Middelbrook 7H10 agar

19.0

Bacto Middelbrook 7H10 agar :

Composition	capacity	unit
(NH₄)₂SO_4	0.5	g
KH₂PH₄	1.5	g
Na₂HPO₄	1.5	g
Na-citrate	0.4	g
MgSO₄	0.025	g
CaCl₂	0.5	g
ZnSO₄	1.0	mg
CuSO₄	1.0	mg
L-glutamic acid	0.5	g
Ferric ammonium citrate	0.04	g
Pyridoxine	1.0	mg
Biotin	0.5	mg
Malachite green	0.25	mg
Bacto agar	15.0	g
Distilled water	1000.0	ml

Bacto Middelbrook OADC enrichment :

Composition	capacity	unit
Oleic acid	0.05	g
Albumin fraction V, bovine	5.0	g
Dextrose	2.0	g
Catalase (beef)	4.0	mg
NaCl	0.85	g
Distilled water	100.0	ml

Final pH at 25℃ 6.6 |+| 0.2.

** ph : 6.6

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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