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Media No. 498 CLOSTRIDIUM CELLOBIOPARUM MEDIUM
| Composition | capacity | unit |
|---|---|---|
| K2HPO4 | 0.50 | g |
| (NH4)2SO4 | 0.50 | g |
| KH2PO4 | 0.50 | g |
| NaCl | 1.00 | g |
| Clarified rumen fluid or sludge fluid (see medium 119) | 300.00 | ml |
| Cellobiose or cellulose | 5.00 | g |
| MgSO4·7H2O | 0.10 | g |
| NaHCO3 | 10.00 | g |
| Na2S·9H2O | 0.25 | g |
| CaCl2·2H2O | 0.10 | g |
| Resazurin | 1.00 | mg |
| Distilled water | 700.00 | ml |
| Cysteine-HCl·H2O | 0.25 | g |
** ph : 6.8
** memo : Adjust pH to 6.8. The medium is prepared under 100% CO_2_ atmosphere. Concentrated solutions each of
cellobiose and of the reducing agents are separately autoclaved under nitrogen atmosphere. Cultures
of DSM 1801 will lyse totally when they reach the stationary phase of growth (after ca. 24 h).
** additional actions :