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Media No. 554 AMPHIBACILLUS MEDIUM
| Composition | capacity | unit |
|---|---|---|
| K2HPO4 | 1 | g |
| Glucose | 10 | g |
| NH4NO3 | 2 | g |
| FeSO4·7H2O | 0.005 | g |
| MgSO4·7H2O | 0.2 | g |
| MnSO4· 7H2O | 0.005 | g |
| Polypepton· | 0.3 | g |
| CaCl2·2H2O | 0.1 | g |
| Yeast extract | 3 | g |
| Agar (if needed) | 15 | g |
| Resazurin | 0.001 | g |
** ph : 10
** memo : After autoclaving, adjust pH to 10.0 with sterilized 10% Na_2_CO_3_ solution.
** additional actions :