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Media No. 559 ALICYCLOBACILLUS MEDIUM

Composition

capacity

unit

Solution A:

Composition	capacity	unit
Yeast extract	2.00	g
Glucose	5.00	g
Distilled water (for liquid medium)	1000.00	mg/L
Distilled water (for solid medium)	500.00	mg/L
Adjust pH to 4.0
CaCl₂ . 2H₂O	0.25	g
MgSO₄ . 7H₂O	0.50	g
(NH₄)₂ SO₄	0.20	g
KH₂PO₄	3.00	g

Solution B:

Composition	capacity	unit
Trace element sol. SL-6	1.00	ml

Solution C:

Composition	capacity	unit
Agar	15.00	g
Distilled water	500.00	ml

Trace element solution SL-6:

Composition	capacity	unit
Distilled water	1000.00	ml
ZnSO₄ . 7H₂O	0.10	g
CoCl₂ . 6H₂O	0.20	g
NiCl₂ . 6H₂O	0.02	g
MnCl₂ . 4H₂O	0.03	g
H₃BO₃	0.30	g
CuCl₂ . 2H₂O	0.01	g
Na₂MoO₄ . 2H₂O	0.03	g

Sterilize separately. For liquid medium combine solution A (with 1000.0 ml distilled water) and solution B. For solid medium combine solution A (with 500 ml distilled water), solution B and solution C. For strains of A. cycloheptanicus add 5 g/l of yeast extract instead of 2 g/l.

** ph : 4

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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